تحميل كتاب RT PCR Protocols PDF - Joe O Connell

تحميل كتاب RT PCR Protocols pdf 2002م - 1443هـ نبذه عن الكتاب: Preface Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imbalances in specific mRNA expression between normal and diseased tissues. Because of its remarkable sensitivity, RT-PCR enables the detection of low-abundance mRNAs even at the level of individual cells. RT-PCR has afforded many opportunities in diagnostics, allowing sensitive detection of RNA viruses such as HIV and HCV. RT-PCR facilitates many diverse techniques in research, including in situ localization of mRNA, antibody engineering, and cDNA cloning. In particular, the present work highlights how RT-PCR complements other technological advances, such as laser-capture microdissection (LCM), real-time PCR, microarray technology, HPLC, and time-resolved fluorimetry .